CD14 Antikörper

Produktnummer ABIN1176993

Dieses Anti-CD14-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD14 in FACS, IP und BR. Geeignet für Maus. Dieses Primary Antibody wurde in 9+ Publikationen zitiert.

Kurzübersicht für CD14 Antikörper (ABIN1176993)

Target: CD14

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Klon: 4C1-CD14

Produktdetails anti-CD14 Antikörper

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse monocyte/macrophage cell line

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: For western blot and flow cytometric analysis, investigators may find Rat Anti-Mouse CD14 mAb rmC5-3 (Cat. No. 553738), FITC Rat Anti-Mouse CD14 (Cat. No. 553739) and/or PE Rat Anti-Mouse CD14 (Cat. No. 553740) to be useful.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CD14 Antikörper (ABIN1176993)

Tasaka, Ishizaka, Yamada, Shimizu, Koh, Hasegawa, Adachi, Yamaguchi: "Effect of CD14 blockade on endotoxin-induced acute lung injury in mice." in: American journal of respiratory cell and molecular biology, Vol. 29, Issue 2, pp. 252-8, (2003) (PubMed).

Akashi, Saitoh, Wakabayashi, Kikuchi, Takamura, Nagai, Kusumoto, Fukase, Kusumoto, Adachi, Kosugi, Miyake: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).

Triantafilou, Triantafilou: "Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster." in: Trends in immunology, Vol. 23, Issue 6, pp. 301-4, (2002) (PubMed).

Nagaoka, Hirota, Niyonsaba, Hirata, Adachi, Tamura, Heumann: "Cathelicidin family of antibacterial peptides CAP18 and CAP11 inhibit the expression of TNF-alpha by blocking the binding of LPS to CD14(+) cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3329-38, (2001) (PubMed).

Yang, Sugawara, Monodane, Nishijima, Adachi, Akashi, Miyake, Hase, Takada: "Micrococcus luteus teichuronic acids activate human and murine monocytic cells in a CD14- and toll-like receptor 4-dependent manner." in: Infection and immunity, Vol. 69, Issue 4, pp. 2025-30, (2001) (PubMed).

Le Roy, Di Padova, Adachi, Glauser, Calandra, Heumann: "Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2759-65, (2001) (PubMed).

Ziegler-Heitbrock: "Heterogeneity of human blood monocytes: the CD14+ CD16+ subpopulation." in: Immunology today, Vol. 17, Issue 9, pp. 424-8, (1997) (PubMed).

Haziot, Ferrero, Köntgen, Hijiya, Yamamoto, Silver, Stewart, Goyert: "Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice." in: Immunity, Vol. 4, Issue 4, pp. 407-14, (1996) (PubMed).

Matsuura, Ishida, Setoguchi, Higuchi, Akizuki, Yamamoto: "Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide." in: The Journal of experimental medicine, Vol. 179, Issue 5, pp. 1671-6, (1994) (PubMed).

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: The 4C1/CD14 antibody reacts with CD14, a ~55-kDa glycosyl phosphatidyl inositol (GPI)-linked cell-surface glycoprotein. Flow cytometric analysis demonstrates that 4C1/CD14 antibody stains CD14 transfectants, the monocyte/macrophage cell line RAW 264.7 peritoneal exudate neutrophils and macrophages, and cultured bone marrow-derived macrophages, but not undifferentiated bone marrow cells, splenocytes, or peripheral blood leukocytes. This staining pattern is similar to that of the alternate anti-mouse CD14 mAb rmC5-3, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils. Therefore, data suggest that CD14 expression by leukocyte populations may differ in mice and humans. CD14 is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein). After binding to LPS-LPB, CD14 associates with integral membrane proteins (such as TLR4-MD-2) which mediate signal transduction. It is involved in the development of endotoxic shock, innate immunity, inflammatory responses, and LPS-stimulated bone resorption. Unlike mAb rmC5-3, the 4C1/CD14 antibody blocks the binding of LPS to CD14.

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades